Novalifetech delivers next day plasmids with puromycin selection
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Puromycin resistance occurred in Escherichia coli after adding the plasmid to the growth medium in the presence of subinhibitory concentrations in the absence of selective pressure, resistances to Puromycin.
This amplification of resistance was observed in laboratory and naturally occurring E. coli strains as well as in polA and recA strains. With the exception of previously identified cmlA and cmlB mutations, Puro resistances were not P1 transducible. Coincident with the emergence of resistance was the appearance of a previously cryptic energy-dependent efflux system. The expression of resistance phenotypes and the puromycin efflux system were temperature sensitive at 42 degrees C.
CKS2 gRNA (BRDN0001146116)
CKS2 gRNA (BRDN0001149143)
GV298-Filamin shRNA Plasmid
HA- HIF1alpha- wt- pBabe- puro
Human Endoplasmic reticulum aminopeptidase 1,ERAP1 ELISA KIT
Human NPEPPS ELISA Kit
Human Puromycin- sensitive aminopeptidase, NPEPPS ELISA KIT