Puromycin selection plasmids

Novalifetech delivers next day plasmids with puromycin selection

Please contact us for pricing

Puromycin resistance occurred in Escherichia coli after adding the plasmid to the growth medium in the presence of subinhibitory concentrations in the absence of selective pressure, resistances to Puromycin.

This amplification of resistance was observed in laboratory and naturally occurring E. coli strains as well as in polA and recA strains. With the exception of previously identified cmlA and cmlB mutations, Puro resistances were not P1 transducible. Coincident with the emergence of resistance was the appearance of a previously cryptic energy-dependent efflux system. The expression of resistance phenotypes and the puromycin efflux system were temperature sensitive at 42 degrees C.